未分类

TRPM2离子通道是感受温暖所需的通道

热激活的离子通道能检测到几乎所有层级的自然温度,例如extreme heat(TRPV2),painful heat(TRPV1,TRPM3,ANO1),non-painful heat(TRPV3,TRPV4),non-painful coolness(TRPM8),painful cold(TRPA1)。

Thermal rangeChannels
extreme heatTRPV2
painful heatTRPV1,TRPM3,ANO1
non-painful heatTRPV3,TRPV4
non-painful coolnessTRPM8
painful coldTRPA1
不同TRP channel感受的温度范围

但是,除了TRPM8之外,如果只是简单地敲除上表中单个离子通道并不会对实验动物热反应有太多影响。Chun-Hsiang Tan在他发表的The TRPM2 ion channel is required for sensitivity to warmth 中,使用钙成像确认了一系列神经元,这些神经元不表达任何已知的TRP 通道。然后使用钙成像,电生理,RNA seq确认这个产生热感的离子通道为TRPM2。自主神经也能表达TRPM2并且能够直接感受到热量。TRPM2敲除的小鼠对non-nouxious warm的感觉显著降低。

在对dorsal root ganlia(被神经节,DRG)使用已知的TRP激活剂进行激活并进行钙成像后,作者发现有大约10%的神经元对这些激活剂并不敏感,但只对温度的变化敏感。这也许存在一个全新的热敏神经元,能感受到34-42°C区间的温度。

Fig. 1a 使用已知离子通道的激活剂进行刺激和使用热进行刺激后,使用fura-2(F340/380)对钙离子的检测。

Fig. 1a 中,从上到下分别为表达TRPV1的神经元,能对辣椒素(Caps)做出反应;第二行是表达TRPM3的神经元,对pregenolone sulphate (PS, blue)能做出反应;第三行是TRPV1和TRPM3共同表达的神经元;第四行就是那个新发现的,对已知诱导剂不敏感的Trp通道。最下面一行为温度,从以上可以看出,这个新的离子通道对已有的诱导剂不敏感,而对温度敏感。对这中新型的神经元进行加热,可以看到他的温度阈值(Fig. 1d)

Fig 1.d 在DRG中发现的新型热敏神经元的温度感受阈值。可以明显看到该神经元在34以上才被激活。下图为逐渐提升的温度,上图为随着温度提升而变化的F340/380 比例

另外,在敲除了小鼠的 Trpm2 基因后,从Trpm2 -/- 小鼠中取出的该种对诱导剂不敏感的神经元显著对42度以上高温的敏感性显著降低。(Fig.1 e)

Fig1. e

持续更新中。。。。。

53 thoughts on “TRPM2离子通道是感受温暖所需的通道

  1. Because its beginning, his company has grown to end
    up being a Multi-Million Dollar Web Advertising and Media Company that specializes in SEO (seo ).
    Kotton Grammer is likewise an active partner of another business that goes by the name where he has trained over 10,000 individuals in Web marketing considering that
    2014 Including myself Kotton has also sold over 25 million dollars
    of digital products through webinars because 2014.

  2. I’ve been browsing online more than 2 hours today, yet
    I never found any interesting article like yours.
    It’s pretty worth enough for me. In my opinion, if all web owners and bloggers made good content as you
    did, the internet will be a lot more useful
    than ever before.

  3. Howdy! Someone in my Myspace group shared this website with us so I came to give it a look.
    I'm definitely loving the information. I'm bookmarking and
    will be tweeting this to my followers! Superb blog
    and fantastic style and design.

  4. I’ve been surfing online more than three hours today,
    yet I never found any interesting article like yours. It is pretty worth
    enough for me. Personally, if all webmasters and bloggers made good content as you did,
    the web will be much more useful than ever before.

  5. It is the best time to make some plans for the future and it
    is time to be happy. I have read this publish and if I may just I wish to recommend you few fascinating things
    or suggestions. Maybe you could write next articles regarding this
    article. I want to read more issues approximately it!

  6. It’s the best time to make some plans for the future and it’s
    time to be happy. I have read this post and if I could I want to
    suggest you few interesting things or tips. Perhaps you could
    write next articles referring to this article. I wish to read more
    things about it!

  7. I’ll immediately grab your rss feed as I can not to find your e-mail subscription link or newsletter service.
    Do you’ve any? Kindly permit me recognize so that I may
    subscribe. Thanks.

  8. It is the best time to make some plans for the future and it’s time to be happy.
    I have read this post and if I could I wish to suggest you
    few interesting things or tips. Maybe you could write next articles
    referring to this article. I desire to read
    even more things about it!

  9. It’s appropriate time to make some plans for the future and it
    is time to be happy. I’ve learn this publish
    and if I may just I wish to counsel you some interesting things or
    advice. Maybe you can write next articles relating to this article.

    I want to learn even more things approximately it!

  10. I simply could not depart your web site before suggesting that I extremely enjoyed the usual info a person provide in your visitors?
    Is gonna be again often in order to check out new posts

  11. I have been surfing online more than 4 hours today, yet I never found any
    interesting article like yours. It's pretty worth enough for me.
    Personally, if all site owners and bloggers made good content as you did, the web will be a lot more useful than ever before.

  12. I have been browsing online more than three hours today, but I by
    no means discovered any attention-grabbing article like yours.

    It is lovely worth sufficient for me. Personally, if all
    site owners and bloggers made good content material
    as you did, the web will be much more helpful than ever before.

发表评论

邮箱地址不会被公开。